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Every free peptide calculator on this site, in one place.

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A growing library of free, independently useful peptide and chemistry calculators, grouped by category.

Molecular & Mass (11)
Average Molecular Weight Calculator Adds each residue's average mass from a pasted sequence plus one water for the two free termini, giving the peptide's average molecular weight. Educational reference, not medical or dosing advice. Molecular & Mass Monoisotopic Mass Calculator Adds each residue's monoisotopic mass from a pasted sequence plus one monoisotopic water, giving the peptide's exact monoisotopic mass. Educational reference, not medical or dosing advice. Molecular & Mass Molecular Formula Calculator Sums the free amino acid formula for each residue and removes (n-1) waters for the peptide bonds to output the peptide's molecular formula. Educational reference, not medical or dosing advice. Molecular & Mass Molarity Calculator Calculates molarity as M = (mass / molar mass) / volume from a mass, unit, molar mass and volume you supply. Educational reference, not medical or dosing advice. Molecular & Mass Mass to Moles Calculator Converts a mass and molar mass into moles (mol, mmol, umol, nmol or pmol), the reverse of moles-to-mass. Educational reference, not medical or dosing advice. Molecular & Mass Moles to Mass Calculator Converts an amount in moles and a molar mass into mass (g, mg, ug or ng), the reverse of mass-to-moles. Educational reference, not medical or dosing advice. Molecular & Mass Concentration from Mass Calculator Divides a mass by a volume, in your chosen units, to give concentration in g/L and mg/mL. Educational reference, not medical or dosing advice. Molecular & Mass Molar Mass Cross-Check Calculator Compares a supplied molecular weight against the mass derived from the sequence itself, reporting the absolute and percent difference. Educational reference, not medical or dosing advice. Molecular & Mass Mass Difference Calculator Reports the absolute and percent difference between two masses, useful for comparing before and after a modification or two batches. Educational reference, not medical or dosing advice. Molecular & Mass Modification Mass Calculator Adds the UniMod monoisotopic mass delta for a chosen modification (acetylation, amidation, phosphorylation, oxidation or deamidation) to a base mass. Educational reference, not medical or dosing advice. Molecular & Mass Terminal Modification Calculator Adds independent, UniMod-referenced mass deltas for the N-terminus (free amine, acetylation, or pyroglutamate formation from Gln or Glu) and the C-terminus (free acid or amidation) to a base mass. Educational reference, not medical or dosing advice. Molecular & Mass
Sequence Analysis (17)
Amino Acid Composition Calculator Counts each amino acid in a pasted sequence and reports its share of the total as a percentage. Educational reference, not medical advice. Sequence Analysis Peptide Length Calculator Counts the number of residues in a pasted amino acid sequence. An educational reference tool only, not medical or dosing advice. Sequence Analysis Net Charge Calculator Sums Henderson-Hasselbalch charge contributions from the termini and ionizable side chains (Bjellqvist pKa values) at a chosen pH. Educational reference, not medical advice. Sequence Analysis Isoelectric Point (pI) Calculator Finds the isoelectric point by bisection, the pH where the same net-charge sum used by the net charge calculator equals zero. Educational reference, not medical advice. Sequence Analysis Charge vs pH Curve Calculator Samples net charge across the full pH 0-14 range using the Henderson-Hasselbalch method, for plotting a charge-versus-pH curve. Educational reference, not medical advice. Sequence Analysis GRAVY Hydrophobicity Calculator Averages the Kyte-Doolittle hydropathy value of every residue in a sequence to give its GRAVY score. Educational reference, not medical advice. Sequence Analysis Hydrophobic Residue Fraction Calculator Reports the percentage of residues classed nonpolar or aromatic by side-chain type. Educational reference, not medical advice. Sequence Analysis Polar Residue Fraction Calculator Reports the percentage of residues classed as polar by side-chain type. Educational reference, not medical advice. Sequence Analysis Charged Residue Fraction Calculator Reports the percentage of residues classed acidic or basic by side-chain type. Educational reference, not medical advice. Sequence Analysis Aromaticity Calculator Reports the percentage of residues classed aromatic by side-chain type (Phe, Trp, Tyr). Educational reference, not medical advice. Sequence Analysis Aliphatic Index Calculator Computes the Ikai aliphatic index, Ala% + 2.9 x Val% + 3.9 x (Ile% + Leu%), a measure of thermostability. Educational reference, not medical advice. Sequence Analysis Boman Index Calculator Averages the negative transfer free energy of each residue (Boman's method) to estimate protein-binding potential in kcal/mol. Educational reference, not medical advice. Sequence Analysis Extinction Coefficient Calculator Computes molar extinction coefficient at 280 nm as 5500 x Trp + 1490 x Tyr + 125 x cystine bonds (Gill and von Hippel / Pace method). Educational reference, not medical advice. Sequence Analysis Beer-Lambert Absorbance Calculator Applies the Beer-Lambert law, A = epsilon x c x l, to compute absorbance from extinction coefficient, concentration and path length. Educational reference, not medical advice. Sequence Analysis Beer-Lambert Concentration Calculator Rearranges the Beer-Lambert law to solve for concentration, c = A / (epsilon x l), from absorbance and path length. Educational reference, not medical advice. Sequence Analysis Beer-Lambert Path Length Calculator Rearranges the Beer-Lambert law to solve for path length, l = A / (epsilon x c), from absorbance and concentration. Educational reference, not medical advice. Sequence Analysis Mass to Sequence Estimator Estimates an approximate residue count from a target mass using the mean residue mass across the 20 standard amino acids; many sequences share one mass. Educational reference, not medical advice. Sequence Analysis
Concentration & Dilution (10)
C1V1 Dilution Calculator Solves the dilution equation C1V1 = C2V2 for whichever one of the four values you leave blank. For lab and research use; not medical or dosing advice. Concentration & Dilution Concentration Unit Converter Converts a concentration between mass-per-volume units: g/L, mg/L, mg/mL, ug/mL and ng/mL. For lab and research use; not medical or dosing advice. Concentration & Dilution Molar Concentration Converter Converts a concentration between molar units: M, mM, uM, nM and pM. For lab and research use; not medical or dosing advice. Concentration & Dilution Mass-Molarity Converter Converts a concentration between a mass-per-volume unit (g/L, mg/L, mg/mL, ug/mL, ng/mL) and a molar unit (M, mM, uM, nM, pM) using a molar mass you supply. For lab and research use; not medical or dosing advice. Concentration & Dilution Stock Solution Calculator Computes the mass to weigh out as target concentration (mg/mL) multiplied by the final volume (mL). For lab and research use; not medical or dosing advice. Concentration & Dilution Working Solution Calculator Solves C1V1 = C2V2 for the stock volume needed, then reports the diluent volume that completes the final volume. For lab and research use; not medical or dosing advice. Concentration & Dilution Serial Dilution Calculator Applies a fixed dilution factor across N steps, concentration(i) = initial / factor^i, and reports diluent volume per transfer. For lab and research use; not medical or dosing advice. Concentration & Dilution Concentration After Volume Change Calculator Recomputes concentration after adding or removing volume with no solute change: new C = (C1 x V1) / (V1 + change). For lab and research use; not medical or dosing advice. Concentration & Dilution Buffer Dilution Calculator Solves the same C1V1 = C2V2 relationship as the working solution calculator, framed in buffer x-strength units (e.g. 10x to 1x). For lab and research use; not medical or dosing advice. Concentration & Dilution Dilution Factor Calculator Divides an initial value by a final value to give the fold dilution applied, shown as both a number and a 1:X ratio. For lab and research use; not medical or dosing advice. Concentration & Dilution
Laboratory Preparation (6)
Pharmacokinetic Models (10)
Elimination Rate Constant Calculator Converts a half-life into a first-order elimination rate constant, k = ln(2) / half-life. A textbook model, not medical or dosing advice. Pharmacokinetic Models Half-Life from Rate Constant Calculator Converts a first-order elimination rate constant into a half-life, half-life = ln(2) / k, the inverse of k-from-half-life. A textbook model, not medical or dosing advice. Pharmacokinetic Models First-Order Elimination Calculator Applies the first-order decay model A(t) = A0 x e^(-kt) to compute the amount and fraction remaining after time t. A textbook model, not medical or dosing advice. Pharmacokinetic Models Time to Percent Remaining Calculator Solves the first-order decay model for elapsed time, t = half-life x log2(1 / fraction remaining), given a target percent. A textbook model, not medical or dosing advice. Pharmacokinetic Models Percent Remaining Calculator Applies the first-order decay model to compute the percent remaining after a given elapsed time and half-life. A textbook model, not medical or dosing advice. Pharmacokinetic Models Accumulation Factor Calculator Computes the repeat-dosing accumulation factor R = 1 / (1 - e^(-k x tau)) for a half-life and fixed dosing interval tau. A textbook model, not medical or dosing advice. Pharmacokinetic Models Time to Steady State Calculator Finds the half-lives (and time) needed to reach a chosen percent of the steady-state asymptote, n = log2(1 / (1 - target fraction)). A textbook model, not medical or dosing advice. Pharmacokinetic Models Steady State Fraction Calculator Computes the fraction of steady state reached after N evenly spaced doses, 1 - (e^(-k x tau))^N, given half-life and interval. A textbook model, not medical or dosing advice. Pharmacokinetic Models Time to Target Amount Calculator Solves the first-order decay model for the time needed to fall from an initial amount to a target amount, t = half-life x log2(A0 / target). A textbook model, not medical or dosing advice. Pharmacokinetic Models Effective Half-Life Calculator Combines two elimination half-lives with the reciprocal-sum rule, 1 / t_eff = 1 / tA + 1 / tB. A textbook model, not medical or dosing advice. Pharmacokinetic Models
Cost & Inventory (6)
Unit Converters (6)